Beyond Single-Cell Analysis of Metallodrugs by ICP-MS: Targeting Cellular Substructures.

DOI
Publisher DOI
PubMed ID
34502377
Abstract
Platinum compounds such as cisplatin (cisPt) embody the backbone of combination chemotherapy protocols against advanced lung cancer. However, their efficacy is primarily limited by inherent or acquired platinum resistance, the origin of which has not been fully elucidated yet, although of paramount interest. Using single cell inductively coupled plasma mass spectrometry (SC-ICP-MS), this study quantifies cisPt in single cancer cells and for the first time in isolated nuclei. A comparison of cisPt uptake was performed between a wild type (wt) cancer cell line and related resistant sublines. In both, resistant cells, wt cells, and their nuclei, cisPt uptake was measured at different incubation times. A lower amount of cisPt was found in resistant cell lines and their nuclei compared to wt cells. Moreover, the abundance of internalized cisPt decreased with increasing resistance. Interestingly, concentrations of cisPt found within the nuclei were higher than compared to cellular concentrations. Here, we show, that SC-ICP-MS allows precise and accurate quantification of metallodrugs in both single cells and cell organelles such as nuclei. These findings pave the way for future applications investigating the potency and efficacy of novel metallodrugs developed for cancer treatment.
Date Issued
2021-08-31
Publication Type
Article
Subject(s)
Subjects
NSCLC chemotherapy cisplatin cisplatin resistance nuclear uptake single cell ICP-MS
Language(s)
en
Author(s)
Galé, Audrey
Hofmann, Lukas
Additional Credits
Journal
International journal of molecular sciences
Publisher
MDPI
ISSN
1422-0067
Access(Rights)
open.access
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