Comparative expression patterns of selected membrane transporters in differentiated BeWo and human primary trophoblast cells.

DOI
Publisher DOI
PubMed ID
30501881
Abstract
Although placental membrane transporters have an important impact on materno-fetal nutrient transfer, placental cell models are poorly characterized regarding transporter expression. We assessed the mRNA expression of 26 physiologically important solute carriers and ABC transporters in BeWo (b30 clone) and primary human trophoblast cells (PHT) before and after syncytialization. 77% of the transporters showed similar mRNA expression changes between BeWo and PHT after syncytialization. Selected transporters, however, were either lacking in BeWo or showed different trends after syncytialization. In conclusion, BeWo cells generally represent an apt model for transporter studies, but their suitability should be confirmed for each transporter by comparison with PHT.
Date Issued
2018-12
Publication Type
Article
Subject(s)
Subjects
ABC transporter BeWo b30 clone Cell fusion Forskolin Placenta SLC transporter Syncytialization Trophoblast
Language(s)
en
Author(s)
Moser-Haessig, Ruedi
Additional Credits
Journal
Placenta
Publisher
Elsevier
ISSN
0143-4004
Access(Rights)
restricted
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