Human mast cells express intracellular TRAIL
Publisher DOI
PubMed ID
20189551
Abstract
Recently we demonstrated that human mast cells (MC) express functional TRAIL death receptors. Here we assessed the expression of TRAIL on both mRNA and protein level in cord blood derived MC (CBMC) and HMC-1. The TRAIL release either spontaneous or induced by LPS, IFN-gamma and IgE-dependent activation, was evaluated as well. The protein location was restricted to the intracellular compartment in CBMC, but not in HMC-1. The intracellular TRAIL was not localized inside the granules. The treatment with IFN-gamma and LPS up-regulated intracellular TRAIL expression in CBMC, but did not induce its release. These in vitro data show that human MC can produce and express intracellular TRAIL whose location could not be altered by different stimuli.
Date Issued
2010
Publication Type
Article
Language(s)
en
Author(s)
Berent-Maoz, Beata | |
Mankuta, David | |
Levi-Schaffer, Francesca |
Additional Credits
Journal
Cellular immunology
Publisher
Elsevier
ISSN
0008-8749
Access(Rights)
metadata.only