Publication:
Cell population, viability, and some key immunomodulatory molecules in different milk somatic cell samples in dairy cows

Simple item page
cris.virtual.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
cris.virtual.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
cris.virtual.author-orcid0000-0002-5817-3428
cris.virtualsource.author-orcid55c65727-ec18-4e99-b38f-fc1aaed1c621
cris.virtualsource.author-orcid576e1b0c-6ec0-412f-b7a1-25842ad408eb
cris.virtualsource.author-orcidd9c7a78c-12ce-42ba-9d13-7215c4c490d9
dc.contributor.authorBaumert, Amandine
dc.contributor.authorBruckmaier, Rupert
dc.contributor.authorWellnitz, Olga
dc.date.accessioned2024-10-14T10:28:13Z
dc.date.available2024-10-14T10:28:13Z
dc.date.issued2009
dc.description.abstractImmune cells in the milk are most important in combating pathogens that invade the mammary gland. This study investigated the immune competence and viability of somatic milk cells that are already resident in milk and udders free of infection. Cells were studied in freshly removed milk to simulate conditions in the udder. Effects of incubation, cell preparation, and immunological stimulation with 0.5 mug/ml lipopolysaccharide (LPS) from Escherichia coli were analysed. Viability and differential counts of milk cells between high and low somatic cell count (SCC) quarters, and cisternal and alveolar milk with and without LPS stimulation were compared. Incubation and preparation of cells caused a cell loss which further increased with time independently of SCC and milk fraction. The viability of these cells was stable until 3 h post incubation and decreased until 6 h. Cell populations differed between both investigations, but did not change during the course of the experiment. mRNA expression of immune and apoptosis factors of the cells, measured by qPCR, did not change substantially: mRNA expression of caspase 3, Toll like receptor 4, and GM-CSF did not change, whereas the expression of the death receptor Fas/APO-1 (CD95), lactoferrin and lysozyme was decreased at 6 h. Cyclooxygenase-2 and TNF-alpha mRNA expression were decreased after 6 h of LPS treatment. In comparison with other studies in vivo or in vitro (in cell culture), in this study where cells are studied ex vivo (removed from the udder but kept in their natural environment, the milk) resident milk cells seem to be more vulnerable, less viable, less able to respond to stimulation, and thus less immune competent compared with cells that have freshly migrated from blood into milk after pathogen stimulation. The cell viability and differential cell count differed between high- and low-SCC milk and between cisternal and alveolar milk depending on the individual cow. In conclusion, the results support the view that for a most effective defence against invading pathogens the mammary gland is reliant on the recruitment of fresh immune cells from the blood.
dc.description.numberOfPages9
dc.description.sponsorshipDepartment of Clinical Research and Veterinary Public Health, Veterinär-Physiologie
dc.identifier.doi10.7892/boris.38376
dc.identifier.isi000269415300015
dc.identifier.publisherDOI10.1017/S0022029909004129
dc.identifier.urihttps://boris-portal.unibe.ch/handle/20.500.12422/111558
dc.language.isoen
dc.publisherCambridge University Press
dc.publisher.placeLondon
dc.relation.ispartofJournal of dairy research
dc.relation.issn0022-0299
dc.relation.organizationDCD5A442C0BCE17DE0405C82790C4DE2
dc.titleCell population, viability, and some key immunomodulatory molecules in different milk somatic cell samples in dairy cows
dc.typearticle
dspace.entity.typePublication
dspace.file.typetext
oaire.citation.endPage64
oaire.citation.issue3
oaire.citation.startPage356
oaire.citation.volume76
oairecerif.author.affiliationDepartment of Clinical Research and Veterinary Public Health, Veterinär-Physiologie
oairecerif.author.affiliationDepartment of Clinical Research and Veterinary Public Health, Veterinär-Physiologie
oairecerif.author.affiliationDepartment of Clinical Research and Veterinary Public Health, Veterinär-Physiologie
oairecerif.author.affiliation2#PLACEHOLDER_PARENT_METADATA_VALUE#
oairecerif.author.affiliation2#PLACEHOLDER_PARENT_METADATA_VALUE#
oairecerif.author.affiliation2#PLACEHOLDER_PARENT_METADATA_VALUE#
oairecerif.author.affiliation3#PLACEHOLDER_PARENT_METADATA_VALUE#
oairecerif.author.affiliation3#PLACEHOLDER_PARENT_METADATA_VALUE#
oairecerif.author.affiliation3#PLACEHOLDER_PARENT_METADATA_VALUE#
oairecerif.author.affiliation4#PLACEHOLDER_PARENT_METADATA_VALUE#
oairecerif.author.affiliation4#PLACEHOLDER_PARENT_METADATA_VALUE#
oairecerif.author.affiliation4#PLACEHOLDER_PARENT_METADATA_VALUE#
unibe.contributor.rolecreator
unibe.contributor.rolecreator
unibe.contributor.rolecreator
unibe.date.licenseChanged2019-10-25 13:26:53
unibe.description.ispublishedpub
unibe.eprints.legacyId38376
unibe.journal.abbrevTitleJ DAIRY RES
unibe.refereedTRUE
unibe.subtype.articlejournal

Files

Original bundle
Now showing 1 - 1 of 1
Name:
S0022029909004129.pdf
Size:
126.5 KB
Format:
Adobe Portable Document Format
File Type:
text
License:
publisher
Content:
published
Download

Collections

Publications