Improved protocol for chromatofocusing on the ProteomeLab PF2D
Publisher DOI
PubMed ID
16941567
Abstract
Beckman-Coulter has recently introduced the ProteomeLab PF2D for 2-D liquid separation of protein samples. The system features separation in the first dimension by chromatofocusing, followed by RP chromatography in the second dimension, allowing the analysis of complex proteomics samples. When used by the standard protocol, reproducibility and column life times are limited, making the use of the instrument very costly. We here present an improved protocol for chromatofocusing, which enhances column life by at least fivefold.
Date Issued
2006
Publication Type
Article
Language(s)
en
Additional Credits
Journal
Proteomics
Publisher
Wiley-VCH
ISSN
1615-9853
ISBN
16941567
Access(Rights)
metadata.only