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  3. Duration-dependent influence of dynamic torsion on the intervertebral disc: an intact disc organ culture study.
 

Duration-dependent influence of dynamic torsion on the intervertebral disc: an intact disc organ culture study.

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BORIS DOI
10.7892/boris.73191
Date of Publication
November 2015
Publication Type
Article
Division/Institute

Institut für chirurgi...

Author
Chan, Samantha C W
Walser, Jochen
Ferguson, Stephen J
Gantenbein, Benjaminorcid-logo
Institut für chirurgische Technologien und Biomechanik (ISTB)
Subject(s)

500 - Science::570 - ...

600 - Technology::610...

Series
European spine journal
ISSN or ISBN (if monograph)
0940-6719
Publisher
Springer
Language
English
Publisher DOI
10.1007/s00586-015-4140-6
PubMed ID
26215177
Uncontrolled Keywords

Bioreactor

Complex loading

Dynamic loading

Intervertebral disc

Organ culture

Torsion

Description
PURPOSE

Mechanical loading is an important parameter that alters the homeostasis of the intervertebral disc (IVD). Studies have demonstrated the role of compression in altering the cellular metabolism, anabolic and catabolic events of the disc, but little is known how complex loading such as torsion-compression affects the IVD cell metabolism and matrix homeostasis. Studying how the duration of torsion affects disc matrix turnover could provide guidelines to prevent overuse injury to the disc and suggest possible beneficial effect of torsion. The aim of the study was to evaluate the biological response of the IVD to different durations of torsional loading.

METHODS

Intact bovine caudal IVD were isolated for organ culture in a bioreactor. Different daily durations of torsion were applied over 7 days at a physiological magnitude (±2°) in combination with 0.2 MPa compression, at a frequency of 1 Hz.

RESULTS

Nucleus pulpous (NP) cell viability and total disc volume decreased with 8 h of torsion-compression per day. Gene expression analysis suggested a down-regulated MMP13 with increased time of torsion. 1 and 4 h per day torsion-compression tended to increase the glycosaminoglycans/hydroxyproline ratio in the NP tissue group.

CONCLUSIONS

Our result suggests that load duration thresholds exist in both torsion and compression with an optimal load duration capable of promoting matrix synthesis and overloading can be harmful to disc cells. Future research is required to evaluate the specific mechanisms for these observed effects.
Handle
https://boris-portal.unibe.ch/handle/20.500.12422/136083
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art%3A10.1007%2Fs00586-015-4140-6.pdftextAdobe PDF1.26 MBpublisherpublishedOpen
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