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A cloned classical swine fever virus derived from the vaccine strain GPE- causes cytopathic effect in CPK-NS cells via type-I interferon-dependent necroptosis.

cris.virtualsource.author-orcid9949857e-0795-43e8-916e-08020ec60b0b
cris.virtualsource.author-orcid585fa9ba-f543-42cc-9617-a879f790176b
cris.virtualsource.author-orcid14bcea6e-c224-4fc8-95a3-01d8aec94cb1
dc.contributor.authorItakura, Yukari
dc.contributor.authorMatsuno, Keita
dc.contributor.authorIto, Asako
dc.contributor.authorGerber, Markus Daniel
dc.contributor.authorLiniger, Matthias
dc.contributor.authorFujimoto, Yuri
dc.contributor.authorTamura, Tomokazu
dc.contributor.authorKameyama, Ken-Ichiro
dc.contributor.authorOkamatsu, Masatoshi
dc.contributor.authorRuggli, Nicolas
dc.contributor.authorKida, Hiroshi
dc.contributor.authorSakoda, Yoshihiro
dc.date.accessioned2024-10-05T09:53:10Z
dc.date.available2024-10-05T09:53:10Z
dc.date.issued2020-01-15
dc.description.abstractClassical swine fever viruses (CSFVs) do typically not show cytopathic effect (CPE) in cell culture, while some strains such as vaccine strain the GPE- induce CPE in the swine kidney-derived CPK-NS cell line cultured in serum-free medium. These latter strains commonly lack Npro-mediated inhibition of type-I interferon (IFN) induction. In order to explore the molecular mechanisms of GPE--induced CPE, we analyzed the cellular pathways involved. In CPK-NS cells infected with the attenuated-vaccine-derived vGPE- strain, both, apoptosis and necroptosis were induced. Necroptosis was type-I IFN-dependent and critical for visible CPE. In contrast, the parental virulent vALD-A76 strain did not induce any of these pathways nor CPE. We used reverse genetics to investigate which viral factors regulate these cell-death pathways. Interestingly, a mutant vGPE- in which the Npro function was restored to inhibit type-I IFN induction did not induce necroptosis nor CPE but still induced apoptosis, while an Npro-mutant vALD-A76 incapable of inhibiting type-I IFN production induced necroptosis and CPE. Although Erns of CSFV is reportedly involved in controlling apoptosis, apoptosis induction by vGPE- or apoptosis inhibition by vALD-A76 were independent of the unique amino acid difference found in Erns of these two strains. Altogether, these results demonstrate that type-I IFN-dependent necroptosis related to non-functional Npro is the main mechanism for CPE induction by vGPE-, and that viral factor(s) other than Erns may induce or inhibit apoptosis in vGPE- or vALD-A76 infected CPK-NS cells, respectively.
dc.description.sponsorshipInstitut für Virologie und Immunologie (IVI)
dc.identifier.doi10.7892/boris.145421
dc.identifier.pmid31715204
dc.identifier.publisherDOI10.1016/j.virusres.2019.197809
dc.identifier.urihttps://boris-portal.unibe.ch/handle/20.500.12422/55129
dc.language.isoen
dc.publisherElsevier
dc.relation.ispartofVirus research
dc.relation.issn0168-1702
dc.relation.organizationDCD5A442C0BAE17DE0405C82790C4DE2
dc.subjectApoptosis Classical swine fever virus Necroptosis Type-I interferon
dc.subject.ddc600 - Technology::630 - Agriculture
dc.subject.ddc500 - Science::570 - Life sciences; biology
dc.titleA cloned classical swine fever virus derived from the vaccine strain GPE- causes cytopathic effect in CPK-NS cells via type-I interferon-dependent necroptosis.
dc.typearticle
dspace.entity.typePublication
dspace.file.typetext
oaire.citation.startPage197809
oaire.citation.volume276
oairecerif.author.affiliationInstitut für Virologie und Immunologie (IVI)
oairecerif.author.affiliationInstitut für Virologie und Immunologie (IVI)
oairecerif.author.affiliationInstitut für Virologie und Immunologie (IVI)
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unibe.date.licenseChanged2020-07-30 06:24:47
unibe.description.ispublishedpub
unibe.eprints.legacyId145421
unibe.journal.abbrevTitleVIRUS RES
unibe.refereedTRUE
unibe.subtype.articlejournal

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