Publication: A cloned classical swine fever virus derived from the vaccine strain GPE- causes cytopathic effect in CPK-NS cells via type-I interferon-dependent necroptosis.
cris.virtualsource.author-orcid | 9949857e-0795-43e8-916e-08020ec60b0b | |
cris.virtualsource.author-orcid | 585fa9ba-f543-42cc-9617-a879f790176b | |
cris.virtualsource.author-orcid | 14bcea6e-c224-4fc8-95a3-01d8aec94cb1 | |
dc.contributor.author | Itakura, Yukari | |
dc.contributor.author | Matsuno, Keita | |
dc.contributor.author | Ito, Asako | |
dc.contributor.author | Gerber, Markus Daniel | |
dc.contributor.author | Liniger, Matthias | |
dc.contributor.author | Fujimoto, Yuri | |
dc.contributor.author | Tamura, Tomokazu | |
dc.contributor.author | Kameyama, Ken-Ichiro | |
dc.contributor.author | Okamatsu, Masatoshi | |
dc.contributor.author | Ruggli, Nicolas | |
dc.contributor.author | Kida, Hiroshi | |
dc.contributor.author | Sakoda, Yoshihiro | |
dc.date.accessioned | 2024-10-05T09:53:10Z | |
dc.date.available | 2024-10-05T09:53:10Z | |
dc.date.issued | 2020-01-15 | |
dc.description.abstract | Classical swine fever viruses (CSFVs) do typically not show cytopathic effect (CPE) in cell culture, while some strains such as vaccine strain the GPE- induce CPE in the swine kidney-derived CPK-NS cell line cultured in serum-free medium. These latter strains commonly lack Npro-mediated inhibition of type-I interferon (IFN) induction. In order to explore the molecular mechanisms of GPE--induced CPE, we analyzed the cellular pathways involved. In CPK-NS cells infected with the attenuated-vaccine-derived vGPE- strain, both, apoptosis and necroptosis were induced. Necroptosis was type-I IFN-dependent and critical for visible CPE. In contrast, the parental virulent vALD-A76 strain did not induce any of these pathways nor CPE. We used reverse genetics to investigate which viral factors regulate these cell-death pathways. Interestingly, a mutant vGPE- in which the Npro function was restored to inhibit type-I IFN induction did not induce necroptosis nor CPE but still induced apoptosis, while an Npro-mutant vALD-A76 incapable of inhibiting type-I IFN production induced necroptosis and CPE. Although Erns of CSFV is reportedly involved in controlling apoptosis, apoptosis induction by vGPE- or apoptosis inhibition by vALD-A76 were independent of the unique amino acid difference found in Erns of these two strains. Altogether, these results demonstrate that type-I IFN-dependent necroptosis related to non-functional Npro is the main mechanism for CPE induction by vGPE-, and that viral factor(s) other than Erns may induce or inhibit apoptosis in vGPE- or vALD-A76 infected CPK-NS cells, respectively. | |
dc.description.sponsorship | Institut für Virologie und Immunologie (IVI) | |
dc.identifier.doi | 10.7892/boris.145421 | |
dc.identifier.pmid | 31715204 | |
dc.identifier.publisherDOI | 10.1016/j.virusres.2019.197809 | |
dc.identifier.uri | https://boris-portal.unibe.ch/handle/20.500.12422/55129 | |
dc.language.iso | en | |
dc.publisher | Elsevier | |
dc.relation.ispartof | Virus research | |
dc.relation.issn | 0168-1702 | |
dc.relation.organization | DCD5A442C0BAE17DE0405C82790C4DE2 | |
dc.subject | Apoptosis Classical swine fever virus Necroptosis Type-I interferon | |
dc.subject.ddc | 600 - Technology::630 - Agriculture | |
dc.subject.ddc | 500 - Science::570 - Life sciences; biology | |
dc.title | A cloned classical swine fever virus derived from the vaccine strain GPE- causes cytopathic effect in CPK-NS cells via type-I interferon-dependent necroptosis. | |
dc.type | article | |
dspace.entity.type | Publication | |
dspace.file.type | text | |
oaire.citation.startPage | 197809 | |
oaire.citation.volume | 276 | |
oairecerif.author.affiliation | Institut für Virologie und Immunologie (IVI) | |
oairecerif.author.affiliation | Institut für Virologie und Immunologie (IVI) | |
oairecerif.author.affiliation | Institut für Virologie und Immunologie (IVI) | |
unibe.contributor.role | creator | |
unibe.contributor.role | creator | |
unibe.contributor.role | creator | |
unibe.contributor.role | creator | |
unibe.contributor.role | creator | |
unibe.contributor.role | creator | |
unibe.contributor.role | creator | |
unibe.contributor.role | creator | |
unibe.contributor.role | creator | |
unibe.contributor.role | creator | |
unibe.contributor.role | creator | |
unibe.contributor.role | creator | |
unibe.date.licenseChanged | 2020-07-30 06:24:47 | |
unibe.description.ispublished | pub | |
unibe.eprints.legacyId | 145421 | |
unibe.journal.abbrevTitle | VIRUS RES | |
unibe.refereed | TRUE | |
unibe.subtype.article | journal |
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