APOSTEL-R Recommendations for Reporting Retinal Optical Coherence Tomography Studies in Rodents.
Publisher DOI
PubMed ID
41061181
Abstract
Background And Objectives
Retinal optical coherence tomography (OCT) in rodent models has been used to longitudinally image retinal changes, to define end points for more costly or time-consuming experiments, and to better understand the pathophysiology underlying OCT findings in human diseases. No standardization of rodent OCT reporting currently exists. Here, we aim to establish consensus recommendation for reporting results from retinal OCT studies in rodents.Methods
Initial recommendations were developed based on the APOSTEL criteria for quantitative OCT reporting in humans by a core team. Using a modified Delphi process, an expert panel of rodent OCT researchers (N = 31) and the wider scientific community discussed, refined, and voted on these initial recommendations. The list of recommendations was then revised and approved by the expert panel.Results
The final 7-point checklist includes reporting recommendations regarding the study protocol, OCT device, acquisition settings and modifications, scanning protocol, funduscopic imaging, postacquisition data selection and image data analyses, and qualitative and quantitative results. With a median agreement score of 3 or 4 out of 4, the scientific community agreed with these recommendations. After revisions, the expert panel accepted the final recommendations.Discussion
The Advised Protocol for OCT Study Terminology and Elements for reporting OCT studies in rodents (APOSTEL-R) originates from an expert consensus. They will provide guidance throughout the experimental process and will contribute to the standardization and quality improvement of preclinical OCT studies.
Retinal optical coherence tomography (OCT) in rodent models has been used to longitudinally image retinal changes, to define end points for more costly or time-consuming experiments, and to better understand the pathophysiology underlying OCT findings in human diseases. No standardization of rodent OCT reporting currently exists. Here, we aim to establish consensus recommendation for reporting results from retinal OCT studies in rodents.Methods
Initial recommendations were developed based on the APOSTEL criteria for quantitative OCT reporting in humans by a core team. Using a modified Delphi process, an expert panel of rodent OCT researchers (N = 31) and the wider scientific community discussed, refined, and voted on these initial recommendations. The list of recommendations was then revised and approved by the expert panel.Results
The final 7-point checklist includes reporting recommendations regarding the study protocol, OCT device, acquisition settings and modifications, scanning protocol, funduscopic imaging, postacquisition data selection and image data analyses, and qualitative and quantitative results. With a median agreement score of 3 or 4 out of 4, the scientific community agreed with these recommendations. After revisions, the expert panel accepted the final recommendations.Discussion
The Advised Protocol for OCT Study Terminology and Elements for reporting OCT studies in rodents (APOSTEL-R) originates from an expert consensus. They will provide guidance throughout the experimental process and will contribute to the standardization and quality improvement of preclinical OCT studies.
Date Issued
2025-11
Publication Type
Article
Subject(s)
Language(s)
en
Author(s)
Oertel, Frederike Cosima | |
Cabrera Debuc, Delia | |
Calabresi, Peter A | |
Chen, Mei | |
Cordano, Christian | |
Dietrich, Michael | |
Feltgen, Nicolas | |
Gramlich, Oliver | |
Green, Ari J | |
Groh, Janos | |
Huang, Su-Chun | |
Knier, Benjamin | |
Korn, Thomas | |
Leocani, Letizia | |
Loewenstein, Anat | |
van Oterendorp, Christian | |
Peto, Tunde | |
Schippling, Sven | |
Schmetterer, Leopold | |
Schmitz-Valckenberg, Steffen | |
Seeliger, Mathias W | |
Shindler, Kenneth S | |
Schuman, Joel S | |
Sindi, Mustafa | |
Tufail, Adnan | |
Wang, Jui-Kai | |
You, Yuyi | |
Lagrèze, Wolf | |
Albrecht, Philipp |
Additional Credits
Journal
Neurology, Neuroimmunology & Neuroinflammation
Publisher
Lippincott, Williams & Wilkins
ISSN
2332-7812
Access(Rights)
open.access