Small intestinal resident eosinophils maintain gut homeostasis following microbial colonization.
Publisher DOI
PubMed ID
35709757
Abstract
The intestine harbors a large population of resident eosinophils, yet the function of intestinal eosinophils has not been explored. Flow cytometry and whole-mount imaging identified eosinophils residing in the lamina propria along the length of the intestine prior to postnatal microbial colonization. Microscopy, transcriptomic analysis, and mass spectrometry of intestinal tissue revealed villus blunting, altered extracellular matrix, decreased epithelial cell turnover, increased gastrointestinal motility, and decreased lipid absorption in eosinophil-deficient mice. Mechanistically, intestinal epithelial cells released IL-33 in a microbiota-dependent manner, which led to eosinophil activation. The colonization of germ-free mice demonstrated that eosinophil activation in response to microbes regulated villous size alterations, macrophage maturation, epithelial barrier integrity, and intestinal transit. Collectively, our findings demonstrate a critical role for eosinophils in facilitating the mutualistic interactions between the host and microbiota and provide a rationale for the functional significance of their early life recruitment in the small intestine.
Date Issued
2022-07-12
Publication Type
Article
Subject(s)
Subjects
eosinophil extracellular matrix germ-free intestinal barrier microbiome small intestine tissue homeostasis villous atrophy
Language(s)
en
Author(s)
Ignacio, Aline | |
Shah, Kathleen | |
Bernier-Latmani, Jeremiah | |
Coakley, Gillian | |
Moyat, Mati | |
Hamelin, Romain | |
Armand, Florence | |
Wong, Nick C | |
Ramay, Hena | |
Thomson, Carolyn A | |
Burkhard, Regula | |
Wang, Haozhe | |
Dufour, Antoine | |
Geuking, Markus B | |
McDonald, Braedon | |
Petrova, Tatiana V | |
Harris, Nicola L | |
McCoy, Kathy D |
Journal
Immunity
Publisher
Elsevier
ISSN
1097-4180
Access(Rights)
restricted