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Publication:
Whatman® FTA® Cards Performance for Ornithobacterium rhinotracheale DNA Amplification

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cris.virtual.author-orcid0000-0001-8554-790X
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cris.virtualsource.author-orcid01ff3924-8cb9-41d3-96ac-8f05e3270ebd
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cris.virtualsource.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
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dc.contributor.authorBerenguer Veiga, Inês Margarida
dc.contributor.authorMühldorfer, Kristin
dc.contributor.authorHafez, Hafez Mohamed
dc.contributor.authorLüschow, Dörte
dc.date.accessioned2024-09-02T17:00:44Z
dc.date.available2024-09-02T17:00:44Z
dc.date.issued2020
dc.description.abstractThe avian pathogen Ornithobacterium rhinotracheale (ORT) has been implied in the etiology of poultry respiratory disease in recent years. To evaluate whether Whatmant Flinders Technology Associates (FTAt) cards can be used for hazard-free transport and storage of ORT samples for posterior DNA amplification, a controlled assay was performed. Three 10-fold dilutions of an ORT culture suspension were spotted on FTA cards and stored at room temperature (RT) for 6 mo. Sterile swabs were immersed in the same three 10-fold culture dilutions and stored at RT and 4 and 20 C without storage medium for the same time. DNA was extracted from both the FTA cards and swabs 1 day, 1 and 6 wk, and 6 mo following sample preparation and stored at 20 C. At the end of the experiment, real-time PCR amplification of the 16S ribosomal RNA gene was performed from DNA extracted throughout a 6-mo period from all ORT samples stored on both FTA cards and swabs. The obtained threshold cycle values for each ORT DNA extraction date were within the same range for all samples in a dilution-dependent fashion, regardless of storage temperature or used material. Pure ORT colonies could be reisolated 1 day after sample preparation from the swab dilutions stored at all temperatures but not from the FTA cards. We conclude that the efficiency of ORT DNA amplification from samples stored on FTA cards or in swabs is similar. However, FTA cards have the advantage of preventing microorganism growth, thus allowing safe transport and storage, for at least 6 mo, for bacterial dilutions down to at least 104 –105 colony-forming units/ml.
dc.description.numberOfPages3
dc.description.sponsorshipInstitut für Tierpathologie (ITPA)
dc.identifier.doi10.48350/152984
dc.identifier.pmid33570099
dc.identifier.publisherDOI10.1637/aviandiseases-D20-00030
dc.identifier.urihttps://boris-portal.unibe.ch/handle/20.500.12422/40402
dc.language.isoen
dc.publisherAmerican Association of Avian Pathologists
dc.relation.ispartofAvian diseases
dc.relation.issn0005-2086
dc.relation.organizationDCD5A442C072E17DE0405C82790C4DE2
dc.relation.organizationDCD5A442C1CCE17DE0405C82790C4DE2
dc.subject.ddc500 - Science::570 - Life sciences; biology
dc.subject.ddc500 - Science::590 - Animals (Zoology)
dc.subject.ddc600 - Technology::630 - Agriculture
dc.titleWhatman® FTA® Cards Performance for Ornithobacterium rhinotracheale DNA Amplification
dc.typearticle
dspace.entity.typePublication
oaire.citation.endPage498
oaire.citation.issue4
oaire.citation.startPage496
oaire.citation.volume64
oairecerif.author.affiliationInstitut für Tierpathologie (ITPA)
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oairecerif.identifier.urlhttps://meridian.allenpress.com/avian-diseases
unibe.contributor.rolecreator
unibe.contributor.rolecreator
unibe.contributor.rolecreator
unibe.contributor.rolecreator
unibe.date.licenseChanged2021-03-12 10:27:42
unibe.description.ispublishedpub
unibe.eprints.legacyId152984
unibe.refereedTRUE
unibe.subtype.articlejournal

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