Six domesticated PiggyBac transposases together carry out programmed DNA elimination in .
Publisher DOI
PubMed ID
30223944
Abstract
The domestication of transposable elements has repeatedly occurred during evolution and domesticated transposases have often been implicated in programmed genome rearrangements, as remarkably illustrated in ciliates. In , PiggyMac (Pgm), a domesticated PiggyBac transposase, carries out developmentally programmed DNA elimination, including the precise excision of tens of thousands of gene-interrupting germline Internal Eliminated Sequences (IESs). Here, we report the discovery of five groups of distant Pgm-like proteins (PgmLs), all able to interact with Pgm and essential for its nuclear localization and IES excision genome-wide. Unlike Pgm, PgmLs lack a conserved catalytic site, suggesting that they rather have an architectural function within a multi-component excision complex embedding Pgm. PgmL depletion can increase erroneous targeting of residual Pgm-mediated DNA cleavage, indicating that PgmLs contribute to accurately position the complex on IES ends. DNA rearrangements in constitute a rare example of a biological process jointly managed by six distinct domesticated transposases.
Date Issued
2018-09-18
Publication Type
Article
Subject(s)
Subjects
DNA elimination IES chromosomes ciliates gene expression piggyBacv transposées domestication
Language(s)
en
Author(s)
Bischerour, Julien | |
Denby Wilkes, Cyril | |
Régnier, Vinciane | |
Mathy, Nathalie | |
Dubois, Emeline | |
Arnaiz, Olivier | |
Sperling, Linda | |
Bétermier, Mireille |
Additional Credits
Journal
eLife
Publisher
eLife Sciences Publications
ISSN
2050-084X
Access(Rights)
open.access