A protocol for generating germ-free Heligmosomoides polygyrus bakeri larvae for gnotobiotic helminth infection studies.
Publisher DOI
PubMed ID
34825215
Abstract
The microbes indigenous to helminth species are a major obstacle to deciphering host-parasite interactions. Repurposing a system of reversible bacterial colonization, we have generated germ-free Heligomosomoides polygyrus bakeri (Hpb) larvae that maintain the sterility of axenic mice upon infection. This protocol provides a valuable tool for controlled studies of helminth-microbiota-immune interactions.
Date Issued
2021-12-17
Publication Type
Article
Subject(s)
Subjects
Cell culture Health Sciences Immunology Microbiology
Language(s)
en
Author(s)
Russell, Gabriel A | |
Peng, Garrie | |
Faubert, Cynthia | |
Verdu, Elena F | |
King, Irah L |
Additional Credits
Journal
STAR protocols
Publisher
Elsevier
ISSN
2666-1667
Access(Rights)
open.access