Tackling feline infectious peritonitis via reverse genetics
Publisher DOI
PubMed ID
25482087
Abstract
Feline infectious peritonitis (FIP) is caused by feline coronaviruses (FCoVs) and represents one of the most important lethal infectious diseases of cats. To date, there is no efficacious prevention and treatment, and our limited knowledge on FIP pathogenesis is mainly based on analysis of experiments with field isolates. In a recent study, we reported a promising approach to study FIP pathogenesis using reverse genetics. We generated a set of recombinant FCoVs and investigated their pathogenicity in vivo. The set included the type I FCoV strain Black, a type I FCoV strain Black with restored accessory gene 7b, two chimeric type I/type II FCoVs and the highly pathogenic type II FCoV strain 79-1146. All recombinant FCoVs and the reference strain isolates were found to establish productive infections in cats. While none of the type I FCoVs and chimeric FCoVs induced FIP, the recombinant type II FCoV strain 79-1146 was as pathogenic as the parental isolate. Interestingly, an intact ORF 3c was confirmed to be restored in all viruses (re)isolated from FIP-diseased animals.
Date Issued
2014
Publication Type
Article
Subject(s)
Subjects
feline coronavirus
•
feline infectious peritonitis
•
reverse genetics
•
recombinant coronaviruses
Language(s)
en
Author(s)
Thiel, Heinz-Jürgen | |
Tekes, Gergely |
Additional Credits
Journal
Bioengineered
Publisher
Taylor & Francis
ISSN
2165-5987
Access(Rights)
metadata.only