Plagl1 regulates the retinal progenitor cell to Müller glial cell transition.

DOI
Publisher DOI
PubMed ID
41849358
Abstract
Müller glia arise from late-stage retinal progenitor cells (RPCs) as a distinct lineage that diverges from neurogenic trajectories. Here, we identify the maternally imprinted gene Plagl1 as a key transcriptional regulator of gliogenesis in the murine retina. Plagl1 is expressed during the RPC-to-glia transition and is dynamically regulated in Müller glia following injury. To define its developmental role, we analyzed Plagl1⁺/⁻pat null mutant retinas at postnatal day 7 (P7), when central retinal gliogenesis is complete. In the absence of Plagl1, Sox9 ⁺ glial/precursor cells were displaced and proliferated ectopically, with structural dysmorphologies, reactive gliosis, and impaired visual processing persisting into later postnatal stages. Bulk RNA-seq and ATAC-seq revealed widespread reductions in chromatin accessibility and transcriptional dysregulation affecting epigenetic modifiers, translational machinery, fate-specifying transcription factors, cell cycle regulators, and signaling pathways. Single-cell pseudobulk analysis showed that Plagl1 loss disrupts chromatin, transcriptional, and translational programs specifically within Sox9 ⁺ cells, encompassing Müller glia and precursor populations, pinpointing these cells as the source of defects in Plagl1⁺/⁻pat retinas. Notch signaling was elevated in Plagl1-deficient glia, and genetic activation at P14 displaced Sox9 ⁺ glial cells, without inducing proliferation. Similarly, conditional deletion of Plagl1 in postnatal Müller glia at P14 disrupted positioning and not cell cycle exit, confirming a cell-autonomous requirement for Müller glia positioning that is independent of proliferation control. Since these conditional manipulations could only be performed at P14 at the earliest, they reveal Plagl1's later functions in postmitotic glia and complement, rather than mirror, the earlier P7 mixed RPC/glial null phenotype. Together these findings establish Plagl1 as a critical regulator of the late-stage RPC to Müller glia transition, acting through coordinated control of chromatin accessibility and gene expression programs to ensure timely cell cycle exit. This function aligns with Plagl1's broader tumor suppressor role in stabilizing postmitotic, differentiated cell states across tissues.
Date Issued
2026-03-18
Publication Type
Article
Subject(s)
Language(s)
en
Author(s)
Touahri, Yacine
Pak, Alissa
David, Luke Ajay
Hanna, Joseph
Liu, Hedy
Xiao, Yucheng
Belfiore, Lauren
Ilnytskyy, Yaroslav
van Oosten, Edwin
Tachibana, Nobuhiko
Adnani, Lata
Zhao, Jiayi
Hoffman, Mary
Dixit, Rajiv
Zinyk, Dawn
Guidos, Cynthia J
Bi, Pengpeng
Aubert, Isabelle
Journot, Laurent
Kovalchuk, Igor
Sauvé, Yves
Biernaskie, Jeff
Wang, Chao
Okawa, Satoshi
Del Sol, Antonio
Schuurmans, Carol
Additional Credits
Journal
PLoS Genetics
Publisher
Public Library of Science
ISSN
1553-7404
1553-7390
Access(Rights)
open.access
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