Synthesis and cellular activity of stereochemically-pure 2’-O-(2-methoxyethyl)-phosphorothioate oligonucleotides

DOI
Publisher DOI
PubMed ID
27966701
Abstract
Stereochemically-pure 2’-O-(2-methoxyethyl)-phosphorothioate (PS-MOE) oligonucleotides were synthesized from new chiral oxazaphospholidine-containing nucleosides. Thermal stability studies showed that the incorporation of Rp-PS linkages increased RNA-binding affinity. In cells, a full Rp-PS-MOE splice-switching oligonucleotide targeting part of the ferrochelatase gene was more potent than its Sp-PS counterpart, but of similar potency to the stereorandom PS-parent sequence.
Date Issued
2017-01-11
Publication Type
Article
Subject(s)
Language(s)
en
Author(s)
Li, Meiling
Lightfoot, Helen Louise
Halloy, Francois
Malinowska, Anna
Berk, Christian
Behera, Alok
Hall, Jonathan
Additional Credits
Journal
Chemical communications
Publisher
Royal Society of Chemistry
ISSN
1359-7345
Access(Rights)
restricted
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