Detection of Basophils and Other Granulocytes in Induced Sputum by Flow Cytometry.
Publisher DOI
PubMed ID
32766984
Abstract
Flow cytometry is one of the most widely used techniques for the detection of surface markers on various cells, particularly the cells of the immune system, at a single-cell resolution. Modern flow cytometers can identify rare cell population in highly heterogeneous samples. Here we present a protocol that allows a precise detection of basophils as well as eosinophils and neutrophils in induced sputum samples. The identification of sputum basophils and other granulocytes contributes to a better understanding of the cellular network that promotes and regulates inflammation of the lower respiratory tract.
Date Issued
2020-08-07
Publication Type
Article
Subjects
Airway inflammation Allergy Asthma Basophils Eosinophils Flow cytometry Induced sputum Neutrophils
Language(s)
en
Additional Credits
Journal
Methods in molecular biology
Publisher
Springer
ISSN
1940-6029
ISBN
978-1-07-160695-7
Book Title
Access(Rights)
restricted