Salivary Hemoglobin Protects against Erosive Tooth Wear in Gastric Reflux Patients.

DOI
Publisher DOI
PubMed ID
33171464
Abstract
OBJECTIVE

Saliva is the most important biological factor to protect against erosive tooth wear (ETW). Gastroesophageal reflux disease (GERD) patients have an increased risk of ETW due to the frequent presence of intrinsic acids in the oral cavity. Remarkably, not all GERD patients suffer from ETW, which might be due to differences in the composition of the saliva.

METHODS

This study compared the proteomic profile of saliva in patients (1) with GERD and ETW (basic erosive wear examination, BEWE, score ≥9; GE group) and (2) with GERD without ETW (BEWE = 0; GNE group) using shotgun label-free quantitative proteomic analysis nLC-ESI-MS/MS. The ability of hemoglobin (Hb) to protect against initial enamel erosion caused by a daily 10-s immersion of enamel specimens in 0.01 M HCl (pH 2.3) for 3 days was evaluated in vitro for proof of concept. Surface hardness change was used as response variable.

RESULTS

The differential expression of Hb subunits was significantly increased in the GNE group versus the GE group, in particular the Hb α-subunit that showed a >22-fold increase. Expressions of serum albumin (4.5-fold) and isoforms of cytoskeletal keratin type II (>3-fold) were also increased in the GNE group. Proteinase inhibitors, such as α1-antitrypsin and α2-macroglobulin, were only identified in the GNE group. In vitro, Hb (1.0 and 4.0 mg/mL) significantly reduced initial enamel erosion compared to a negative control after 3 days.

CONCLUSIONS

Our results indicate that many proteins, with special emphasis on Hb, may be involved in the resistance of GERD patients to the occurrence of ETW. These proteins may be candidates for inclusion in dental products to protect against ETW.
Date Issued
2020-11-10
Publication Type
Article
Subject(s)
Subjects
Gastroesophageal reflux Hemoglobins Proteomics Saliva Tooth wear
Language(s)
en
Author(s)
Martini, Tatiana
Rios, Daniela
Dionizio, Aline
Cassiano, Luiza de Paula Silva
Taioqui Pelá, Vinicius
E Silva, Cíntia Maria de Sousa
Taira, Even A
Ventura, Talita Mendes
Magalhães, Ana Carolina
de Oliveira, Ricardo Brandt
Palma-Dibb, Regina Guenka
Buzalaf, Marília Afonso Rabelo
Additional Credits
Journal
Caries research
Publisher
Karger
ISSN
0008-6568
Access(Rights)
restricted
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