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  3. A cysteine protease inhibitor of plasmodium berghei is essential for exo-erythrocytic development
 

A cysteine protease inhibitor of plasmodium berghei is essential for exo-erythrocytic development

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BORIS DOI
10.7892/boris.71013
Date of Publication
August 2014
Publication Type
Article
Division/Institute

Institut für Zellbiol...

Author
Lehmann, Christine
Heitmann, Anna
Mishra, Satish
Burda, Paul-Christian
Institut für Zellbiologie (IZB)
Singer, Mirko
Prado, Monica
Niklaus, Liviaorcid-logo
Institut für Zellbiologie (IZB)
Lacroix, Céline
Ménard, Robert
Frischknecht, Friedrich
Limenitakis, Rebecca Rachel
Institut für Zellbiologie (IZB)
Sinnis, Photini
Heussler, Volkerorcid-logo
Institut für Zellbiologie (IZB)
Subject(s)

500 - Science::570 - ...

Series
PLoS pathogens
ISSN or ISBN (if monograph)
1553-7366
Publisher
Public Library of Science
Language
English
Publisher DOI
10.1371/journal.ppat.1004336
PubMed ID
25166051
Description
Plasmodium parasites express a potent inhibitor of cysteine proteases (ICP) throughout their life cycle. To analyze the role of ICP in different life cycle stages, we generated a stage-specific knockout of the Plasmodium berghei ICP (PbICP). Excision of the pbicb gene occurred in infective sporozoites and resulted in impaired sporozoite invasion of hepatocytes, despite residual PbICP protein being detectable in sporozoites. The vast majority of these parasites invading a cultured hepatocyte cell line did not develop to mature liver stages, but the few that successfully developed hepatic merozoites were able to initiate a blood stage infection in mice. These blood stage parasites, now completely lacking PbICP, exhibited an attenuated phenotype but were able to infect mosquitoes and develop to the oocyst stage. However, PbICP-negative sporozoites liberated from oocysts exhibited defective motility and invaded mosquito salivary glands in low numbers. They were also unable to invade hepatocytes, confirming that control of cysteine protease activity is of critical importance for sporozoites. Importantly, transfection of PbICP-knockout parasites with a pbicp-gfp construct fully reversed these defects. Taken together, in P. berghei this inhibitor of the ICP family is essential for sporozoite motility but also appears to play a role during parasite development in hepatocytes and erythrocytes.
Handle
https://boris-portal.unibe.ch/handle/20.500.12422/134655
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Lehmann_2014.1004336.pdftextAdobe PDF3.23 MBpublishedOpen
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