Molecular anatomy of adult mouse leptomeninges.

DOI
Publisher DOI
PubMed ID
37776854
Abstract
Leptomeninges, consisting of the pia mater and arachnoid, form a connective tissue investment and barrier enclosure of the brain. The exact nature of leptomeningeal cells has long been debated. In this study, we identify five molecularly distinct fibroblast-like transcriptomes in cerebral leptomeninges; link them to anatomically distinct cell types of the pia, inner arachnoid, outer arachnoid barrier, and dural border layer; and contrast them to a sixth fibroblast-like transcriptome present in the choroid plexus and median eminence. Newly identified transcriptional markers enabled molecular characterization of cell types responsible for adherence of arachnoid layers to one another and for the arachnoid barrier. These markers also proved useful in identifying the molecular features of leptomeningeal development, injury, and repair that were preserved or changed after traumatic brain injury. Together, the findings highlight the value of identifying fibroblast transcriptional subsets and their cellular locations toward advancing the understanding of leptomeningeal physiology and pathology.
Date Issued
2023-12-06
Publication Type
Article
Subject(s)
Subjects
arachnoid barrier arachnoid mater brain fibroblasts dura mater leptomeninges perivascular fibroblast pia mater single-cell RNA sequencing traumatic brain injury tricellular junction
Language(s)
en
Author(s)
Pietilä, Riikka
Del Gaudio, Francesca
He, Liqun
Vázquez-Liébanas, Elisa
Vanlandewijck, Michael
Muhl, Lars
Mocci, Giuseppe
Bjørnholm, Katrine D
Lindblad, Caroline
Fletcher-Sandersjöö, Alexander
Svensson, Mikael
Thelin, Eric P
Liu, Jianping
van Voorden, A Jantine
Torres, Monica
Antila, Salli
Karlström, Helena
Storm-Mathisen, Jon
Bergersen, Linda Hildegard
Moggio, Aldo
Hansson, Emil M
Ulvmar, Maria H
Nilsson, Per
Mäkinen, Taija
Andaloussi Mäe, Maarja
Alitalo, Kari
McDonald, Donald M
Lendahl, Urban
Andrae, Johanna
Betsholtz, Christer
Additional Credits
Journal
Neuron
Publisher
Elsevier
ISSN
1097-4199
Access(Rights)
Unknown
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