Immune response of horses to vaccination with the recombinant Hc domain of botulinum neurotoxin types C and D
Publisher DOI
Abstract
Botulinum neurotoxins, predominantly serotypes C and D, cause equine botulism through forage poisoning. The C-terminal part of the heavy chain of botulinum neurotoxin types C and D (HcBoNT/C and D) was expressed in Escherichia coli and evaluated as a recombinant mono- and bivalent vaccine in twelve horses in comparison to a commercially available toxoid vaccine. A three-dose subcutaneous immunization of adult horses elicited robust serum antibody response in an ELISA using the immunogen as a capture antigen. Immune sera showed dose-dependent high potency in neutralizing specifically the active BoNT/C and D in the mouse protection assay. The aluminium hydroxide based mono- and bivalent recombinant HcBoNT/C and D vaccines were characterized by good compatibility and the ability to elicit protective antibody titers similar or superior to the commercially available toxoid vaccine.
Date Issued
2009
Publication Type
Article
Language(s)
en
Author(s)
Mazuet, C. | |
Popoff, M. |
Journal
Vaccine
Publisher
Elsevier
ISSN
0264-410X
Access(Rights)
metadata.only