Chemokines and integrins independently tune actin flow and substrate friction during intranodal migration of T cells.
Publisher DOI
PubMed ID
29777221
Abstract
Although much is known about the physiological framework of T cell motility, and numerous rate-limiting molecules have been identified through loss-of-function approaches, an integrated functional concept of T cell motility is lacking. Here, we used in vivo precision morphometry together with analysis of cytoskeletal dynamics in vitro to deconstruct the basic mechanisms of T cell migration within lymphatic organs. We show that the contributions of the integrin LFA-1 and the chemokine receptor CCR7 are complementary rather than positioned in a linear pathway, as they are during leukocyte extravasation from the blood vasculature. Our data demonstrate that CCR7 controls cortical actin flows, whereas integrins mediate substrate friction that is sufficient to drive locomotion in the absence of considerable surface adhesions and plasma membrane flux.
Date Issued
2018-06
Publication Type
Article
Language(s)
en
Author(s)
Hons, Miroslav | |
Kopf, Aglaja | |
Hauschild, Robert | |
Leithner, Alexander | |
Gaertner, Florian | |
Renkawitz, Jörg | |
Stein, Jens V | |
Sixt, Michael |
Additional Credits
Journal
Nature immunology
Publisher
Nature Publishing Group
ISSN
1529-2908
Access(Rights)
restricted