Affinity retardation chromatography: characterization of the method and its application. The description of low affinity laminin self-interactions.
Publisher DOI
PubMed ID
7856840
Abstract
Affinity retardation chromatography (ARC), a method for the examination of low-affinity interactions, is mathematically described in order to characterize the method itself and to estimate binding coefficients of self-assembly domains of basement membrane protein laminin. Affinity retardation was determined by comparing the elutions on a "binding" and on a "nonreacting" column. It depends on the binding coefficient, the concentrations of both ligands, and the nonbinding elution position. Half maximal binding of the NH2-terminal domain of laminin B1-short arm to the A- and/or B2-short arms was estimated to occur at 10-17 microM for noncooperative and at < or = 3 microM for cooperative binding. A model of the laminin polymerization, postulating two levels of cooperative binding behavior, is described.
Date Issued
1994-10
Publication Type
Article
Subject(s)
Language(s)
en
Additional Credits
Journal
Analytical biochemistry
Publisher
Elsevier
ISSN
0003-2697
Access(Rights)
metadata.only