Quantitative Analysis of Epigenetic Modifications in Fagopyrum Nuclei with Confocal Microscope, ImageJ, and R Studio.
Publisher DOI
PubMed ID
38532089
Abstract
Epigenetic programming plays a vital role in regulating pluripotency genes, which become activated or inactivated during the processes of dedifferentiation and differentiation during an organism's development. The analysis of epigenetic modifications has become possible through the technique of immunostaining, where specific antibodies allow the identification of a single target protein. This chapter describes a detailed protocol for the analysis of the epigenetic modifications with the use of confocal microscopy, subsequent image, and statistical analysis on the example of Fagopyrum calli with the use of nine antibodies raised against histone H3 and H4 methylation and acetylation on several lysines as well as DNA methylation.
Date Issued
2024
Publication Type
Article
Subject(s)
Subjects
Confocal microscopy Epigenetics R Studio
Language(s)
en
Author(s)
Tomasiak, Alicja | |
Sala, Katarzyna | |
Braszewska, Agnieszka |
Additional Credits
Journal
Methods in molecular biology
Publisher
Springer
ISSN
1940-6029
Access(Rights)
restricted