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  3. A 120-y time series of genomes reveals the consequences of closed breeding in German Shepherd Dogs.

A 120-y time series of genomes reveals the consequences of closed breeding in German Shepherd Dogs.

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DOI
10.48620/92708
Publisher DOI
10.1073/pnas.2421755122
PubMed ID
41284896
Abstract
Many contemporary breed dogs display reduced genomic health compared to mixed-breed dogs, including reduced heterozygosity and increased genetic load, likely due to strong directed breeding. Lack of historical genomes, however, has made it difficult to disentangle the timing and drivers of these declines given complex and breed-specific demographic histories, artificial selection, and crossbreeding. Here, we sequenced genomes of nine 20th-century museum specimens (1906-1993) of pedigree German Shepherd Dogs (GSDs) to directly assess the consequences of contemporary breeding practices on genomic diversity and health over time. The GSD breed offers an excellent case study, given the breed's fluctuations in global popularity, differing selective regimes, and the GSD's use in the establishment of other breeds, including wolfdogs. Genome-wide heterozygosity showed significant reductions after the Second World War (WWII), coincident with an increase in both the frequency of runs of homozygosity and load. We also detected repeated population bottlenecks linked to the use of popular sires throughout the 20th century. Finally, although hybridization with wolves has led to genome-wide increases in heterozygosity in wolfdog breeds derived from the GSD, nonadmixed ancestry blocks (dog or wolf) were severely depleted in diversity due to the limited number of founders involved in their establishment. Combined, our results indicate that declines in the genomic health of GSDs and related breeds occurred not at the onset of breed formation but throughout the last century, as a result of population bottlenecks associated with WWII and the repeated use of popular sires.
Date Issued
2025-12-02
Publication Type
Article
Subject(s)
500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health
500 Science > 590 Animals (Zoology)
Subjects
breed
•
canine
•
hybridization
•
inbreeding
•
selection
Language(s)
en
Author(s)
Scarsbrook, Lachie
Spatola, Gabriella J
Dreger, Dayna L
Feuerborn, Tatiana R
Buckley, Reuben M
Carmagnini, Alberto
Fabre, Anne-Claire  
Institute of Ecology and Evolution (IEE)  
Harris, Alexander C
Hertwig, Stefan T.  
Institute of Ecology and Evolution (IEE)  
Leeb, Tosso  
Department of Clinical Research and Veterinary Public Health (DCR-VPH)  
Institute of Genetics  
Mousseau, Timothy A
Tabbada, Kristina
Thalmann, Olaf
Larson, Greger
Frantz, Laurent A F
Ostrander, Elaine A
Additional Credits
Institute of Genetics  
Institute of Ecology and Evolution (IEE)  
Department of Clinical Research and Veterinary Public Health (DCR-VPH)  
Journal
Proceedings of the National Academy of Sciences
Publisher
National Academy of Sciences
ISSN
1091-6490
0027-8424
Access(Rights)
open.access
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