Screening of ethyl methane sulphonate mutagenized tef [ Eragrostis tef (Zucc.) Trotter] population identifies Al-tolerant lines
Publisher DOI
Abstract
About 15,000 M2 seeds of ethyl-methane-sulphonate (EMS)-mutagenized population were screened along with Al-tolerant and sensitive checks and the M0 variety. Strongly acidic soil with an external application of a toxic Al-solution and exposure to moisture stress was used to maximize selection pressure. Twenty-one M2 plants with root lengths of greater than the mean of the tolerant check were selected and planted for seed production. Candidate M3 plants were investigated for Al-tolerance and for morpho-agronomic traits under greenhouse and field conditions, respectively. Highly significant differences were observed for Al-tolerance between the candidate mutant lines and the M0 (P < .001), and between mutant lines and the sensitive check (P < .001). Similarly, significant differences were observed between the mutant lines for 16 of the 20 quantitative traits measured. This study is the first to report successful induction of enhanced Al-tolerance in tef by using EMS mutagenized population.
Date Issued
2017-04-10
Publication Type
Article
Subject(s)
Subjects
aluminium
•
ethyl methane sulphonate (EMS)
•
mutation breeding
•
tef
Language(s)
en
Author(s)
Desta, Ermias A. | |
Hussein, Shimelis | |
Laing, Mark | |
Mengistu, Fentahun |
Additional Credits
Journal
Journal of plant interactions
Publisher
Taylor & Francis
ISSN
1742-9145
Access(Rights)
open.access