Actin dynamics and the Bmp pathway drive apical extrusion of proepicardial cells

DOI
Publisher DOI
PubMed ID
31175121
Abstract
The epicardium, the outer mesothelial layer enclosing the myocardium, plays key roles in heart development and regeneration. During embryogenesis, the epicardium arises from the proepicardium (PE), a cell cluster that appears in the dorsal pericardium (DP) close to the venous pole of the heart. Little is known about how the PE emerges from the pericardial mesothelium. Using a zebrafish model and a combination of genetic tools, pharmacological agents and quantitative in vivo imaging, we reveal that a coordinated collective movement of DP cells drives PE formation. We found that Bmp signaling and the actomyosin cytoskeleton promote constriction of the DP, which enables PE cells to extrude apically. We provide evidence that cell extrusion, which has been described in the elimination of unfit cells from epithelia and the emergence of hematopoietic stem cells, is also a mechanism for PE cells to exit an organized mesothelium and fulfil their developmental fate to form a new tissue layer, the epicardium.
Date Issued
2019
Publication Type
Article
Subject(s)
Language(s)
en
Author(s)
Andrés-Delgado, Laura
Galardi-Castilla, María
Bazaga, David
Peralta, Marina
Münch, Juliane
González-Rosa, Juan M.
Tessadori, Federico
de la Pompa, José Luis
Vermot, Julien
Additional Credits
Journal
Development
Publisher
Company of Biologists Limited
ISSN
0950-1991
Access(Rights)
open.access
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