Peripheral blood CD163(+) monocytes and soluble CD163 in dry and neovascular age-related macular degeneration
Publisher DOI
PubMed ID
32333612
Abstract
Macrophages are the main infiltrating immune cells in choroidal neovascularization (CNV), a hallmark of the human wet, or neovascular age-related macular degeneration (AMD). Due to their plasticity and ability to adapt to the local microenvironment in a tissue-dependent manner, macrophages display polar functional phenotypes characterized by their cell surface markers and their cytokine profiles. We found accumulation of hemoglobin-scavenging cluster of differentiation 163 (CD163)(+) macrophages in laser-induced CNV lesions and higher expression of CD163(+) monocytes in the peripheral blood on day 7 post injury in mice. In comparison, CD80(+) macrophages did not differ with laser-injury in young or aged mice and did not significantly change in the peripheral blood of CNV mice. We examined the percentages of CD163(+), CD206(+), and CD80(+) monocytes in the peripheral blood of patients with wet AMD, patients with dry AMD, and in age-matched individuals without AMD as controls. Percentages of peripheral blood CD163(+) monocytes in both dry AMD (P < .001) and wet AMD (P < .05) were higher than in age-matched non-AMD controls, while there was no difference between the groups in the percentages of peripheral CD206(+) and CD80(+) monocytes. Further, serum level of soluble CD163 (sCD163) was elevated only in patients with wet AMD (P < .05). An examination of 40 cytokine levels across the study groups revealed that anti-VEGF treated patients with wet AMD, who showed no exudative signs on the day of blood drawing had a cytokine profile that was similar to that of non-AMD individuals. These results indicate that CD163 could be further evaluated for its potential as a useful marker of disease activity in patients with neovascular AMD. Future studies will address the origin and potential mechanistic role of CD163(+) macrophages in wet AMD pathologies of angiogenesis and leakage of blood components.
Date Issued
2020-04-25
Publication Type
Article
Subject(s)
Language(s)
en
Author(s)
Daftarian, Narsis | |
Piryaie, Golbarg | |
Zarif, Mahin Nikougoftar | |
Pirmardan, Ehsan Ranaei | |
Yamaguchi, Muneo | |
Nejad, Qurban Behzadian | |
Hasanpour, Hossein | |
Samiei, Shahram | |
Pfister, Isabel B. | |
Soheili, Zahra‐Soheila | |
Nakao, Shintaro | |
Barakat, Aliaa | |
Garweg, Justus G. | |
Ahmadieh, Hamid | |
Hafezi‐Moghadam, Ali |
Additional Credits
Journal
FASEB journal
Publisher
Federation of American Societies for Experimental Biology
ISSN
0892-6638
Access(Rights)
restricted