CRACM1 is a plasma membrane protein essential for store-operated Ca2+ entry.
Publisher DOI
PubMed ID
16645049
Abstract
Store-operated Ca2+ entry is mediated by Ca2+ release-activated Ca2+ (CRAC) channels following Ca2+ release from intracellular stores. We performed a genome-wide RNA interference (RNAi) screen in Drosophila cells to identify proteins that inhibit store-operated Ca2+ influx. A secondary patch-clamp screen identified CRACM1 and CRACM2 (CRAC modulators 1 and 2) as modulators of Drosophila CRAC currents. We characterized the human ortholog of CRACM1, a plasma membrane-resident protein encoded by gene FLJ14466. Although overexpression of CRACM1 did not affect CRAC currents, RNAi-mediated knockdown disrupted its activation. CRACM1 could be the CRAC channel itself, a subunit of it, or a component of the CRAC signaling machinery.
Date Issued
2006-05-26
Publication Type
Article
Language(s)
en
Author(s)
Vig, M | |
Beck, A | |
Koomoa, D L | |
Rabah, D | |
Koblan-Huberson, M | |
Kraft, S | |
Turner, H | |
Fleig, A | |
Penner, R | |
Kinet, J-P |
Additional Credits
Journal
Science
Publisher
American Association for the Advancement of Science
ISSN
0036-8075
Access(Rights)
restricted