EZH1 Is Associated with TCP-Induced Bone Regeneration through Macrophage Polarization.
Publisher DOI
PubMed ID
30228822
Abstract
Macrophages have been found to regulate the effects of biomaterials throughout the entire tissue repair process as an antigen-presenting cell. As a well-defined osteoconductive biomaterial for bone defect regeneration, tricalcium phosphate (TCP) has been found to facilitate a favourable osteoimmunomodulatory response that can shift macrophage polarization towards the M2 phenotype. In the present study, our group discovered that a histone methyltransferase enhancer of zeste1 (EZH1) was drastically downregulated in Thp1 cells stimulated by TCP, indicating that EZH1 may participate in the macrophage phenotype shifting. Furthermore, the NF-B pathway in macrophages was significantly downregulated through stimulation of TCP, suggesting a potential interaction between EZH1 and the NF-B pathway. Utilizing gene knock-down therapy in macrophages, it was found that depletion of EZH1 induced M2 macrophage polarization but did not downregulate NF-B. When the NF-B pathway was inhibited, the expression of EZH1 was significantly downregulated, suggesting that the inhibition of EZH1 may be regulated by the NF-B pathway. These novel findings provide valuable insights into a potential gene target system that controls M2 macrophage polarization which ultimately favours a microenvironment suitable for bone repair.
Date Issued
2018
Publication Type
Article
Subject(s)
Language(s)
en
Author(s)
Jia, Xiaoshi | |
Xu, Hudi | |
Yin, Chengcheng | |
Zhang, Xiaoxin | |
Wu, Min | |
Zhang, Yufeng |
Additional Credits
Journal
Stem cells international
Publisher
Hindawi
ISSN
1687-966X
Access(Rights)
Unknown