DNA binding properties of oligodeoxynucleotides containing pyrrolidino C-nucleosides
Publisher DOI
PubMed ID
12227767
Abstract
We have incorporated pyrrolidino-C-nucleosides (pyrrolidino-pseudonucleosides) containing the base uracil and N-1-methyl uracil into oligodeoxynucleotides and compared their thermal duplex and triplex stabilities with unmodified or pseudouridine-containing oligodeoxynucleotides. We find relative destabilizations of triplex formation by ca. -13 to -1 degrees C per modification (relative to thymidine) in a strongly sequence dependent mode. Duplex formation is less destabilizing and more homogeneous with -4 to -2 degrees C per modification
Date Issued
2002
Publication Type
Article
Subject(s)
Subjects
base
binding
C-nucleosides
DNA
duplex
DUPLEXES
Oligodeoxynucleotides
properties
pyrrolidino-DNA
stability
thermal
thymidine
Triplex
Uracil
binding
C-nucleosides
DNA
duplex
DUPLEXES
Oligodeoxynucleotides
properties
pyrrolidino-DNA
stability
thermal
thymidine
Triplex
Uracil
Language(s)
en
Author(s)
Additional Credits
Journal
Organic letters
Publisher
American Chemical Society
ISSN
1523-7060
Access(Rights)
restricted