The files contain raw measurement data and normalized data for various treatment/experimental conditions. All mock groups contain the vehicle of the inhibitor used (if any), which is either water, ethanol or DMSO (see in manuscript).

- Animals cytokines.csv - Analysis of the TNFalpha and IL-6 release in brain tissue during bacterial meningitis. Data represents pg/ml from ELISA tests.

- Cytokines D39 Dynasore NOD1.csv - Effect of Nod1 inhibitors and dynamin GTPase inhibitors on the cytokine/chemokine release from primary glial cells in the presence or absence of bacterial lysates. Data represents pg/ml from ELISA tests.

- Cytokines LPS removal.csv - Release of inflammatory cytokines from primary glia before and after the removal of LPS from recombinant pneumolysin. Data represents pg/ml from ELISA tests.

- Cytokines recPLY.csv - Cytokine release from primary glial cells after challenge with recombinant pneumolysin without LPS (polymyxin B-based removal). Data represents pg/ml from ELISA tests.

- Cytokines TLRs.csv - Cytokine release from mixed glial cells upon exposure to pneumoccoccal lysates in the presence of various TLR inhibitors. Data represents pg/ml from ELISA tests.

- Electron microscopy.csv - Counting values of the number of endosomal vesicles, visualized in transmission electron microscopy.

- Endo Astro Micro PLY Dynamin.csv - Inhibition of endocytosis by dynasore and dyngo4a in microglia and astrocytes as judged by FM4-64 assay. Presented are values normalized to timepoint 0, secondarily normalized to the mock curve. Raw data (not shown) is raw fluorescent intensity (0-255 gray levels).

- Endo Astro Micro PLY.csv - Endocytosis enhancement by pneumolysin in microglia and astrocytes. Raw data represents the raw fluorescent intensity (0-255 gray levels), normalized values are nomalized to timepoint 0, secondarily normalized to the mock curve.

- Endo PLY Chlorpromazine.csv - Modulation of pneumolysin-enhanced endocytosis (low and high pneumolysin concentrations) by chlorpromazine in mixed glial cells. Presented are values normalized to timepoint 0, secondarily normalized to the mock curve. Raw data (not shown) is raw fluorescent intensity (0-255 gray levels).

- Endo PLY HEK293.csv - Pneumolysin-enhanced endocytosis in HEK293 cells. Presented are values normalized to timepoint 0, secondarily normalized to the mock curve. Raw data (not shown) is raw fluorescent intensity (0-255 gray levels).

- Endocyto membrane pore insert.csv - Enhanced endocytosis by pneumolysin in the pores of a membrane insert, on which glial cells grow. Raw data represents the raw fluorescent intensity (0-255 gray levels), normalized values are nomalized to timepoint 0, secondarily normalized to the mock curve. 

- Endocytosis all inhibitors data.csv - Modulation of pneumolysin-enhanced endocytosis in mixed glial cells by various inhibitors. Raw data represents the raw fluorescent intensity (0-255 gray levels), normalized values are nomalized to timepoint 0, secondarily normalized to the mock curve.

- Endocytosis D39 dPLY.csv - Endocytosis normalization in mixed glial cells after elimination of pneumolysin expression in pneumococcal lysates (D39 strain). Raw data represents the raw fluorescent intensity (0-255 gray levels), normalized values are nomalized to timepoint 0, secondarily normalized to the mock curve.

- Endocytosis D39 dynamin inhib.csv - Inhibition of the endocytosis enhancement in primary glial cells by pneumococcal lysates (D39 strain) using dynamin inhibitors. Raw data represents the raw fluorescent intensity (0-255 gray levels), normalized values are nomalized to timepoint 0, secondarily normalized to the mock curve. 

- Endocytosis PLY mutants.csv - Importance of the pore-forming capacity of pneumolysin as a critical factor enhancing endocytosis in mixed glial cells. Raw data represents the raw fluorescent intensity (0-255 gray levels), normalized values are nomalized to timepoint 0, secondarily normalized to the mock curve.

- Endocytosis PLY&chol.csv - Inhibition of the pneumolysin-mediated endocytosis by cholesterol preincubation of the toxin. Raw data represents the raw fluorescent intensity (0-255 gray levels), normalized values are nomalized to timepoint 0, secondarily normalized to the mock curve.

- Inhibitor validation.csv - Validation of the activity of inhibitors, which do not alter endocytosis, but inhbit cell motility. The values represent micrometers/min.

- Live imaging overlay multiple.csv - Live imaging follow-up (timescale) of the exosome release, calcium elevation, cell swelling, membrane depolarization and endocytosis in mixed glial cells. Values represent as follows: for swelling - calcein AM fluorescence intensity, inversely normalized to timepoint 0 (increase indicates swelling); Fura-2 - ratio between the fluorescence of both dye fractions (calcium-sensitive and reference), indicating increase in intracellular calcium; DiBac4(3) - normalized (to timepoint 0) fluorescence intensity of the membrane potential-sensitive dye, indicating decreased potential with fluorescent increase; exosome release (Exosomes) - rough count per an image window. 

- Permeability test dynasore.csv - Propidium iodide-based permeability assay after pneumolysin challenge with and without endocytosis inhibition by dynasore. DAPI values represent total number of cells per a field. PI (propidium iodide) data - values with subtracted timepoint 0, secondarily calculated in percentage sung the DAPI total number of cells.

Abbreviations: CFU – colony-forming unit; CPZ - chlorpromazine; BBB – blood–brain barrier; C3 - C3 transferase, RhoA GTPase inhibitor; CSF - cerebrospinal fluid; D39 wt – wild-type Streptococcus pneumoniae strain D39; D39 ∆PLY – Streptococcus pneumoniae strain D39 without pneumolysin expression; DiBac4(3) - bis-(1,3-dibutylbarbituric acid)-trimethine oxonol; FM assay - endocytotic assay using the FMTM 4-64 dye; HU – hemolytic unit; IL-6 – interleukin 6; K-free – potassium-free, LPS – lipopolysaccharide; NLR – Nod-like receptor; NOD – nucleotide-binding oligomerization domain; PAMP – pathogen associated molecular pattern; PI3K – phosphatidylinositol-3-kinase; PIP2– phosphatidylinositol-4,5-biphosphate; and PIP3 - phosphatidylinositol-3,4,5-triphosphate; PKC – protein kinase C; PLC – phospholipase C; PLY – pneumolysin, PRR - pattern recognition receptor; PTEN - phosphatase and tensin homolog phosphatase; RIPK2 – receptor-interacting serine/threonine protein kinase 2; TLR - Toll-like receptor; TNF-alpha - Tumor necrosis factor alpha.

Contact details of the senior author/principal investigator: Dr. Asparouh Iliev, Institute of Anatomy, University of Bern, Baltzerstrasse 2, 3012 Bern, Switzerland, E-mail: asparouh.iliev@unibe.ch, Orcid: 0000-0002-1343-2967.